Figure 2 details the distribution of the 61 samples tested by inter-assay comparison, as well as the distribution of 40 triplicates for each method in the intra-assay evaluation: variability is the cause of frequent results above the threshold of 50 cop/mL, as well as undetected ones.
This work was aimed to the evaluation of the variability of lipolytic activity in Yarrowia lipolytica strains, as well as to asses for a selected strain, the response to the changes of physico-chemical variables (such as pH, NaCl and lipid content), in order to obtain predictive models describing their effects on the lipolysis pattern.
First, data from local weather stations was collected to have a realistic evaluation of the variability of the solar/wind resource at all time scales.
To confirm these results and also for a more precise evaluation of intraspecific variability of satellite DNA copy number, the quantification experiment was repeated by real time qPCR using the same P1-forward/2a-reverse primer pair.
Comparison of gene expression measurements performed by whole-genome microarrays (phase I) and qRT−PCR (phase II) together with evaluation of methodological variability of qRT−PCR is all consistent with previous work (Port et al, 2005; Seidl et al, 2007) and lessens concerns that our findings are due to methodological artifacts.
The authors also measured median and quartiles of the VLA and FBL values for evaluation of variability.
The importance of careful preparation of the friction pair ('bedding-in' and 'burnishing') for brake performance testing and the evaluation of variability by repeat testing is emphasised.
This method was preferable over direct comparison with WT colonies present on the plate as it allowed a more reliable evaluation of variability and this choice was validated by the fact that WT colonies fell close to the average value of the screened population, as shown in Fig. 1b.
The experiment is based on the evaluation of the temporal variability of the strokes according to these 21 measures.
However, despite the growing emphasis on integrated catchment-scale implementation of land-use mitigation measures, the baseline landscape-scale evaluation of the spatial variability of key soil nutrients remains scarce.
