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After incubation, the evaluation of viability and apoptosis was performed.
In the next step they underwent evaluation of viability and functionality.
The total number of spermatozoa recovered by flushing was low and evaluation of viability was limited.
For subsequent dose-depending evaluation of viability, the metabolic activity of cells towards tetrazolium salt XTT was applied according to the manufacturer's instructions (Serva, Heidelberg, Germany).
Flow cytometric evaluation of viability of frozen-thawed PI-stained stallion (5 stallions) spermatozoa correlated with the fertility (r = 0.68) of 40 mares (80 cycles), and was better (p < 0.05) than other methods (MOT, hamster oocyte penetration) [ 90].
By analyzing the viability of metabolisms with decreasing numbers of reactions n, and taking advantage of this relationship, we were able to reduce the computational cost of enumerating viable metabolisms by a factor ≈ 10 to the evaluation of viability for only 1.55 × 10 metabolisms [ 43].
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Barbau-Piednoir, E. et al. Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar Enteritidis.
In experiment 1, the frozen-thawed spermatozoa were stained either with CellTracker Green CMFDA (CT-Green) or CellTracker Red CMPTX (CT-Red), and in vitro evaluations of viability and motility were performed.
Following irradiation, samples were returned to the 37 °C incubator and kept under 5% CO2 for 24 hours before the evaluation of cellular viability with the MTT assay.
Thus, we present an experimental evaluation of the viability of the approach.
Last year, Moscow real estate values soared, the Russian stock market grew by 90percentt and Standard & Poor's upgraded its rating on Russia (its evaluation of the viability of doing business in the country).
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