Sentence examples for evaluation of transcripts from inspiring English sources

Illumina BeadChip probes consist of relatively long (50 mer) oligonucleotides potentially allowing evaluation of transcripts from both species.

As with all RNA-Seq assemblies, careful case-by-case evaluation of transcripts will be advisable given the possibility that highly related genes may have been inadvertently merged [ 47], a particular concern given the substantial expansion of gene families commonly seen in crop plants [ 48, 49].

For the evaluation of transcript expression by quantitative RT-PCR, S. aureus strain N315 was exposed to various stress conditions.

The analysis was repeated twice: on "extended" and "core" subsets of probe-sets and when the probe-set mean was applied for evaluation of transcript cluster expression level.

Evaluation of transcript accuracy was initially based on mapping useful reads of two randomly selected libraries of each sole transcriptome using Bowtie2 (not shown).

Designing specific and effective primers for high-quality, moderate-throughput evaluation of transcript levels, i.e., quantitative, real-time PCR (qPCR), is nontrivial.

We have identified an experimentally validated collection of murine primer pairs for PCR and QPCR which can be used under a common PCR thermal profile, allowing the evaluation of transcript abundance of a large number of genes in parallel.

For robust evaluation of transcript expression level, we eliminated 1101 transcripts that were targeted by less than three unique probes, and we used 508,908 unique probes of 43,934 transcripts in this study (Additional file 26: Table S15).

Our evaluation of transcript levels of genes encoding polysaccharide biosynthesis and cell wall loosening enzymes determined significant transcript reduction for both classes of enzymes in Li 2 mutant elongating fibers.

Evaluation of transcript abundance using real-time PCR was effective to define six time-points when abundance levels of a set of genes representative for the seed filling process varied with respect to their morphology: 18 DAP (torpedo), 20 DAP (bent- cotyledon), 25 DAP (transition bent-embryo fully fills seed) and 30 DAP, 35 DAP and 40 DAP (embryo fully fills the seed).

Thus, the urinary pellet is suitable for evaluation of RNA transcripts and microRNAs, but is limited by transcript abundance [ 23].