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Whereas functional assays involves some kind of re-stimulation, MHC tetramers can be used to identify specific T cells without further in vitro manipulation, and they allow for a simultaneous evaluation of the differentiation state through co-staining for various cell surface differentiation markers.
For evaluation of the differentiation potential, we primed the cells for osteogenic differentiation and detected the presence of osteoblast-like cells by BCIP/NBT staining of alkaline phosphatase.
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These findings suggest that humans have evolved to use neurocomputational architecture dedicated to face processing and reward evaluation for the differentiation of kin, which drives implicit idiosyncratic affectively regulated social interactions.
For evaluation of the osteogenic differentiation of rat bone marrow mesenchymal stem cells, alkaline phosphatase activity and osteogenic gene expression were employed.
For evaluation of the cellular differentiation, confluent HaCaT cells were treated for 48 h at 37 °C with 20 ng/ml KGF or the SNs collected as above.
The study also emphasizes the clinical relevance of the evaluation of tubular differentiation in breast cancer.
Evaluation of the cluster of differentiation antigens present on PI and cured cells verified their common origin from Raji cells (data not shown).
The Gleason score system is a standard applied to assess the histological pattern and spread of PCa [ 10]; Helpap is a method for the histological evaluation of cell differentiation and the existence of atypical prostatic cells [ 11]. cMDX document architecture was designed to meet the Open Packaging Convention (OPC) [ 13].
The evaluation of tumour differentiation was based on histological criteria of the guidelines of the Japanese Society for Esophageal Diseases (Japanese Society for Esophageal Diseases, 1999).
Two criteria scoring system for semiquantitative evaluation of adipogenic differentiation following the oil red O staining procedure.
For the evaluation of osteogenic differentiation, ALP activity was detected by using an ALP kit (Nanjing Jiancheng Technological Inc., Nanjing, China).
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