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To rule out the possibility of any toxic side effects due to uttroside B, a detailed toxicological evaluation of the compound was conducted in Swiss albino mice as shown in Fig. 6(A).
In addition, the evaluation of the compound effects in vivo is especially important when multiple cell types and possibly multiple organs are involved in the toxicologic response.
In vitro and in vivo evaluation of the compound still remains to be elucidated to demonstrate the imaging properties of this RGD derivative.
It was stressed that if new scientific data became available, the JECFA and CCRVDF, as opposed to the Commission, were the appropriate bodies to undertake a new evaluation of the compound.
66. Delegations favouring the adoption of the MRLs without delay stated that the Joint FAO/WHO Expert Committee on Food Additives and Contaminants (JECFA) and the Committee on Residues of Veterinary Drugs in Foods (CCRVDF) had performed a thorough scientific evaluation of the compound and, as additional scientific information had not been provided, a re-evaluation was unnecessary.
While quantitative analysis of BoNT/A efficacy with evaluation of the compound muscle action potential (CMAP) M-wave 7 seems reasonable in neurological diseases, the spatial resolution, in general, is too limited for distinctive monitoring of BoNT/A efficacy in aesthetics.
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Evaluation of the compounds in a radioligand binding assay showed that they lacked affinity to the AT1 receptor.
The in vitro evaluation of the compounds focuses on GST inhibition and stability towards γ-glutamyl-transpeptidase (γGT), the main enzyme involved in GSH breakdown.
Biological evaluation of the compounds shows no discrimination between monomeric and dimeric-ligands in respect to binding affinities, however, the dimeric ligands appear to have different functional properties.
Pharmacological evaluation of the compounds showed that the conformational restriction method can effectively work for improving the pharmacological selectivity of a parent compound and also for investigating the bioactive conformation.
The antiproliferative activity evaluation of the compounds provided evidence that a dimethylamino substituted side chain and the presence of 1,2 double bond play a key role in cell growth inhibition.
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