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However, very few reports have been published to date focused on the evaluation of cell viability of cultured ECs.
Fig. 1 Evaluation of cell viability in MCF-7 cells using cell counting kit (a) and live and dead cell assay (b).
The quantitative evaluation of cell viability was carried out by spectroscopic estimation of formazan (3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium 3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium 3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium 3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium 3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium 3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium
The ATP-based method is highly sensitive, reproducible and simple for the evaluation of cell viability and proliferation [21], [22].
The evaluation of cell viability by CFUs was consistent with biomass quantification obtained by crystal-violet staining.
Evaluation of cell viability was performed by a tetrazolium salt assay using the reagent 3- 4,5-Dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich GmbH).
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Research focused on electrochemical cell chips and the SERS technique have been reviewed here; electrochemical cell chips based on nanostructured surfaces have been developed for the in vitro detection of cell viability and the evaluation of the effects of anticancer drugs, which showed the high capability to evaluate the cytotoxic effects of several chemicals at low concentrations.
It is another indicator of cell viability through the evaluation of membrane integrity.
For quantitative evaluation of protease activity (as a measure of cell viability), luminometry, or fluorescence microscopy, cells were used 24 hr after transfection.
Sensitive detection of cell necrosis is crucial for the determination of cell viability.
The data are presented as percentage of cell viability relative to unstimulated cells (0 μM).
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