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The performance of the method was evaluated as described in the Evaluation of bias correction section.
The studies included were an inhomogeneous group and for this reason the evaluation of bias was omitted.
These datasets are therefore well-suited to be used as reference data for the evaluation of bias factors in sample-based studies.
After calculating the true, observed and corrected areas under the curves, decision errors were assessed using the metrics described in the Evaluation of bias correction section.
The systematic evaluation of bias or risk of bias remains an essential aspect of evidence synthesis, as it forces reviewers to critically examine trials.
For the evaluation of bias correction effects as well as comparison with other methods (Table 1), we used paired-end RNA-seq data from the microarray quality control (MAQC) project (Shi et al., 2006) (Short Read Archive accession number SRA012427), because it contains 907 transcripts which were also analyzed by TaqMan qRT-PCR, out of which 893 matched our reference annotation.
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However, recent evaluations of biases generated in high throughput sequencing data have pinpointed the amplification step as the primary cause [ 4, 5].
The funnel plots for publication bias showed asymmetry for serum SMRP studies (figure 1A) and evaluation of publication bias showed that Egger tests were significant for serum SMRPs (p=0.038).
Looking at the current literature, generally there was a lack of information provided on the selection process of the included and excluded patients, and thus an evaluation of selection bias was compromised.
Especially, there were very poor descriptions and/or implementations regarding the registration (2%), evaluation of publication bias (12%), and appropriate conclusions based on scientific quality of the included studies (27%).
We also used Egger's test and the Begg-Mazumdar test with 95% CI for evaluation of publication bias, which are considered to be significant for P<0.10.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com