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After harvest and phenotypic evaluation, frozen root tissue for all plants in one pot was used for RNA isolation.
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Due to the higher quality of the images, for further evaluation freeze frames from the endoscopic videos were exclusively taken during expiration in all horses.
During routine daily blood testing were performed, samples of serum and urine for NGAL evaluation were frozen; additionally, serum concentrations of cystatin C were evaluated and urine samples for albumin were saved.
Serum for ASP activity evaluation was frozen at −80°C until the analysis was carried out.
Ten days later, the rats were euthanized and the gel foam sponges were removed, washed once with PBS and then either immersion-fixed overnight in 10% buffered formalin for histochemical evaluation or frozen in OCT compound and/or liquid nitrogen for immunofluorescence microscopy or various biochemical and molecular assays.
However, all these evaluations used frozen tissues.
At time of data analysis, a repeated evaluation of the frozen section slides was performed and compared with the original frozen section report.
Samples were assessed macroscopically and representative areas (e.g. tumour nodules) were processed as formalin-fixed, paraffin-embedded blocks for diagnostic evaluation, or snap frozen in liquid nitrogen and stored at −80°C for zymography.
For this evaluation, samples were not frozen and thawed more than once, and we evaluated the samples immediately after collection (at room temperature).
For the diagnosis of PJI, we used serum C-reactive protein (CRP) measurement as a preoperative diagnostic tool, frozen histopathologic evaluation and real-time polymerase chain reaction (PCR) as intraoperative tools, and permanent histopathological evaluation and microbiological culture as postoperative tools.
Turna et al. [9] considered a histological evaluation by intra-operative frozen section and performed complete resection in case of a benign histological results.
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