In cattle, the direct evidence of close associations of CNVR 456 with index of total merit and genetic evaluations for protein production, fat production, and herd life in Holstein had been identified [ 63].
Additionally, the study by Seroussi et al. (2010) indicated there were close associations between CNVR 456, located on BTA18, and index of total merit and genetic evaluations for protein production, fat production, and herd life in Holstein cattle.
In addition to the significant association between the loss of this region and total merit (p < 0.0008), significant associations with copy number were also found with the genetic evaluations for protein production (p < 0.006), fat production (p < 0.001) and herd life (p < 0.007).
Moreover, although CNI can quantitatively evaluate the copy numbers of the ACTN4 gene, our evaluation system for protein expression of actinin-4 cannot quantitatively classify the cases with protein expression of actinin-4.
These data were used only for the evaluation for subcellular protein location, but not used for quantification, nor for PTM analysis, as will be discussed further below.
It has been suggested that the antibodies proposed for evaluation of protein antigens in fixed cells should be tested for signal intensity and specificity by WB. [27] Reliance on WB for evaluation of antibody specificity (or rather, the level of non-specificity) for what is essentially a microscopy application in ICW, presupposes that the proteome is represented identically in both techniques.
We used Q-PCR for quantitative analysis of mRNA level and IHC staining for evaluation of protein expression in cancer tissues.
For evaluation of protein production levels, samples of cell crude extracts were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and stained with copper chloride 0.3 M (Lee et al. 1987).
β-actin was measured on every blot for evaluation of protein loading.
Employing C. elegans expressing GFP-tagged luciferase as a reporter for evaluation of protein stability we show that the chaperoning strategy of body wall muscle cells and neurons is significantly different and that both are differently affected by aging.
