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For functional evaluation, cell lines expressing each construct (as indicated by their acquired resistance to hygromycin treatment following transfection) were evaluated for NAD degradation and their ability to support a TRPM2 dependent Ca2+ transient (Figure 3B).
For functional evaluation, cell populations which exhibited high, low, or negative Lrp5 staining, together with a CD24+CD49fhigh (MRU) fraction for comparison, were sorted into pure fetal bovine serum, centrifuged at 700×g for 5 min, re-suspended in culture media containing 10% DMSO, and frozen in liquid nitrogen prior to transfer into recipient mice.
Figure 3 summarises the criteria for light microscopic evaluation, cell counting and reporting of results.
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For cytotoxicity evaluation, cells were seeded onto 96-well plates in 200 μl of medium for 24 h.
In immunological evaluations, cell counting is generally limited by the underlying quality and structure of the studied tissue sections.
The cells were used for evaluation of cell attachment, cell growth and proliferation.
The TEP examination in vitro was based on the evaluation of cell number, cell phenotype and cell distribution within the scaffold.
To facilitate the evaluation of cell contraction, cells were encapsulated in fibrin gels, and gel diameter was determined after 14 h.
For the evaluation of cell proliferation, relative cell numbers on implant materials were measured (Figure 2).
Antimicrobials' sites of injury were investigated by the evaluation of cell constituents' release, cell surface hydrophobicity and differential scanning calorimetry.
Fig. 1 Evaluation of cell viability in MCF-7 cells using cell counting kit (a) and live and dead cell assay (b).
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