Histopathology was evaluated under a microscope.
Immunostained sections were evaluated under a microscope (Olympus, Japan).
Immunohistochemically stained sections were evaluated under a microscope (Olympus Optical Co. Ltd ,Tokyo, Japan).
The sections were mounted with cover glass and evaluated under a microscope.
Instruments were evaluated under a microscope with 15 × magnifications (Carl Zeiss OPMI Pro Ergo, Germany) for signs of deformation.
Samples were covered with 50 μl Mowiol 4-88® based mounting medium (Calbiochem, Darmstadt) containing 1.6 μl DAPI/ml and evaluated under a microscope (LEICA DM IRE2, Bensheim, Germany).
From this concentrated suspension, a 10 µL aliquot was placed between the blade and the coverslip and evaluated under a microscope (Olympus Corporation, Tokyo, Japan) at 100x magnification.
The distance over which the cells had migrated into the wounded areas following 48 h incubation at 37°C was evaluated under a microscope.
The teratogenicity endpoints (malformation of head, tail, or heart, scoliosis, deformity of yolk, and growth retardation) were evaluated under a microscope at 72 hpf.
When embryos are evaluated under a microscope in an uncontrolled environment, they may suffer from undesirable shock or stress due to sudden changes in known critical parameters including temperature, oxygen concentration and pH [ 15- 19].
