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2) non-specific PCR amplification when evaluating the specificity of the primers using MFEprimer [ 11]; 3) cross-RNAi [ 2] discovered using the BLAST program [ 12] to search for the predicted PCR amplicons against the latest version of the C. elegans genomic DNA sequence; 4) mis-operation, such as sample loading error [ 1], etc.
These are now available for use in hybridoma selection and as reference standards for evaluating the specificity of currently available anti-swine IgG antibodies.
In addition, the present approach may be useful for evaluating the specificity of γ-secretase inhibitors.
In summary, by evaluating the specificity of antibodies to melanoma cells versus melanocytes and fibroblasts we could identify a melanoma-specific monoclonal antibody clone 6_2G3.
We conclude that mice tolerate high levels of CGs rendering them inherently unsuitable for in vivo xenograft experiments evaluating the specificity of cancer cell killing.
Evaluating the specificity of the uptake against detailed clinical investigations of pain presentation, comparisons between DDE and other radioligands (i.e. FDG [30]), a more detailed anatomical resolution (through PET-CT or PET-MRI) and control groups including non-traumatic neck pain and headache patients are warranted.
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Further studies on a broader set of pathosystems are thus required to evaluate the specificity of the involvement of some specific defence mechanisms more accurately.
We used Nox4-specific siRNAs to knockdown endogenous Nox4 to evaluate the specificity of the Nox4 antibody.
HIVwt was also generated in parallel in the presence of the FlAsH dye (HIVwt-F) to evaluate the specificity of the labeling and the potential packaging of non-specifically labeled cellular proteins.
We first evaluated the specificity of the EGFP expression.
Drug free blank hair was analysed to evaluate the specificity of analytical method.
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