The study was designed to examine the effects of model plastic derived compounds, bisphenol A (BPA) and dibutyl phthalate (DBP), on juxtacrine communication in adult rat testis, by evaluating the expression of Notch pathway components.
Quality of the microdissection was determined by evaluating the expression of glomerular specific genes (NPHS1, NPHS2, and SYNPO).
Phenotypic analyses of cytotoxic and helper subsets were performed placing an electronic gate on CD8+ bright and CD4+ bright cells and evaluating the expression of CD45RA versus CCR7.
We analyzed this activity further by evaluating the expression of the alpha-1 and alpha-2 subunits of the Na+/K+-ATPase.
We complemented these studies by evaluating the expression of extracellular matrix and adhesion molecules by a quantitative real-time PCR array.
Here, evaluating the expression of VEGF isoforms, we found that TAT seems to have both angiogenic and lymphangiogenic properties, while SAT seems to be especially an angiogenic source, because VEGF-C expression was mostly detected in TAT.
Since both the PLD isoforms are expressed in BALB/c mice [35], we determined the isoform-specific knockdown of PLD1 by evaluating the expression of PLD2 as well, following the above-mentioned siRNA intravenous administration.
Once observed the elevated number of Treg cells in the peripheral blood of infected donors, we further characterized this cell population by evaluating the expression of molecules and cytokines associated with cell modulation.
A very similar result was obtained when evaluating the expression of HSPB6.
This synergism was further confirmed by evaluating the expression of the Hippo target gene Diap1.
RT-PCR was performed for evaluating the expression of DP and EP receptors.
