Sentence examples for evaluating the activation of from inspiring English sources

In the same cell populations, we studied Src activity by the in vitro kinase assay evaluating the activation of Hck, a member of Src kinases.

Cell survival has been ascertained by using the WST-1 assay and by evaluating the activation of the PI3 kinase/NFκB survival axis.

Aiming at evaluating the activation of coagulation factor XIII, 200  μL of 10 M urea solution was added to the clots and the samples were incubated for 48 h at 37°C.

To determine whether B cells contribute to CD4 T cell activation in the context of active disease, we evaluated the activation of both CD4 T cells and B cells by Leishmania antigens in PBMC cultures.

Using an antibody that specifically recognizes an epitope overlapping the nuclear localization signal of the p65 subunit and, thus, selectively binding to the activated form of p65 (indicated as p65*), we evaluated the activation of NF-κB in single and double transgenic mice at the onset stage by Western blot.

As Shc can also be recruited by IGF-1R activatevate downstream SH2-domain containing proteins, we also evaluated the activation of this alternative pathway.

Having established that BV activates the PI3K-Akt signaling axis [1], we next evaluated the activation of pAkt and pS6 (downstream of mTOR) in response to BV in RAW 264.7 macrophages.

We evaluated the activation of the transcription factor, c-jun, by its phosphorylation on an activating residue, Ser63, as well as the electrophoretic shift caused by the phospho-modification of the protein when Western blots were probed with a total anti-c-jun antibody (Fig. 3A and B).

We then evaluated the activation of JNK1/2 (with the use of specific antibodies that recognize the phosphorylated (activated) form of the kinases), and the expression of the anti-apoptotic protein BAK in epithelial tubular cells collected from these patients.

On the other hand, cyclic voltammetry evaluates the activation of a gold electrode by taking into account the potential difference between the cathodic (E pc) and anodic (E pa) peak currents (ΔE p) (Fig. 1a).

As measures of macrophage function, we evaluated the activation of NF-κB, cytokine production, cell surface marker expression and the phagocytic and antibacterial capabilities of porcine macrophages after in vitro exposure to an organic swine barn dust extract (ODE).