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The subsequent procedures included summarizing data production, evaluating sequencing quality and depth, calculating length distribution of small RNAs, and filtrating contaminated reads.
The raw reads obtained from Solexa sequencing were processed by summarizing data production, evaluating sequencing quality, calculating the length distribution of small RNA reads.
On the other hand, in " miRSeq: a user-friendly standalone toolkit for sequencing quality evaluation and miRNA profiling," C.-T. Pan et al. introduced a new tool for NGS data alignment that not only is easy to implement but also offers various methods for evaluating sequencing quality and provides profiles for up to 105 species for users to compare with.
The raw reads obtained from Solexa sequencing were processed to obtain clean reads by summarizing data production, evaluating sequencing quality, calculating the length distribution of small RNA reads, removing low quality reads and adaptor sequences as described in previous paper [ 41].
Similar(56)
However, current investigation is hampered by the lack of computational methods for systematic evaluating sequence specificity.
The importance of evaluating sequence-related miRNAs during functional analysis has been elegantly exemplified by work on the C. elegans let-7 miRNA family.
RIBOSUM scoring (Bernhart et al., 2008) was added for evaluating sequence similarity.
We removed PPI data redundancies by evaluating sequence similarity and then integrated human PPIs with the H-InvDB proteins.
This composition dependence is important in evaluating sequence conservation across malaria genomes, due to their strong AT bias.
Grouping homologous sequences into different blocks according to the evolutionary distance of the species to human and evaluating sequence conservation in each group independently significantly improved prediction accuracy.
The HCC1187 dataset is a well-recognized baseline dataset from Illumina for evaluating sequence analysis algorithms (Howarth et al., 2011, 2007; Newman et al., 2013).
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