The human eye, combined with appropriate use of the human brain, provides a level of discrimination, which certainly exceeds the ability of the existing vote counting machines to evaluate detached chads.
The number of cells, cultured in 25-cm culture flasks (Falcon, Perugia, Italy) was evaluated by detaching with trypsin solution (0.05% trypsin 0.02% sodium EDTA) and counting using a Bürker chamber and trypan blue solution (0.2% w/ v final dye concentration).
Evaluating CEC directly detached from vessel wall may provide direct insights on the status of the endothelium, as well as providing mechanisms underlying endothelial dysfunction.
Also, the concept is already evaluated in the single family detached house in Japanese conditions [44]. Figure 15a shows that the typical air-conditioning system (Fig. 14a) consumes a large amount of electricity to support the indoor environmental conditions of the house.
By contrast to the classic method that uses detached leaves to evaluate cold tolerance [ 15], we used tissue−cultured grape plants to ensure consistency of plant material for investigating the whole−plant level.
In addition, grip strength of each paw was analyzed by semiquantitatively evaluating the force needed to detach the paw from a wire 3 mm in diameter using a score from 0 to –3 (0, normal grip strength; –1 mildly reduced grip strength; –2 moderately reduced grip strength; –3, severely reduced grip strength).
The functionalities of the cells detached by photoreaction were evaluated.
On day 9 adherent cells were detached, and counted and then evaluated by flow cytometry (30% of cells were CD11c positive and 65% were F4/80 positive).
Typical Philippine single family detached concrete and wooden houses were evaluated based on house occupancy, appliances usage, lighting usage and electricity consumption.
The amount of re-constituted shield was evaluated by measuring the proteins or Alpha GST detached by 50 mM NaCl.
