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The in vitro biocompatibility of the scaffolds was investigated by culturing with HepG2 and MC3T3-E1 cells, and evaluating cell viability with the 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide assay and examining cell morphology by scanning electron and confocal microscopy.
The capacity of 5-FU to induce cell death is abrogated by co-treatment with KGF, whereas in KGFR silenced cells 5-FU efficiently induces cell death even combined to KGF, as determined by evaluating cell viability.
To rule out a possible cell type or species-specific effect for E- or A-Bax mutants, similar studies were performed in human H157 epithelial lung cancer cells using luciferase assay for evaluating cell viability as described [38] [39].
Cortical and cerebellar neurons were treated with or without MG132 for 8 hours (long enough to induce accumulation of SP-PrP in cortical cells), then incubated for 16 hours with or without staurosporine, before evaluating cell viability by MTT assay.
The functional status of intact mitochondria was assessed by evaluating cell viability using MTT [ 18].
MTS [3- 4,5-dimethylthiazol-2-yl -5- 3-carboxymethoxyphenyl -2- 4-sulfophenyl)2 H-tetrazolium, inner salt] assay (Promega, Madison, USA) was used for evaluating cell viability.
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3- 4,5-dimethylthiazol-2yl- -2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate cell viability.
We evaluated cell viability and functions, and also monitored the oxygen consumption.
The methylthiazol tetrazolium (MTT) assay, a cell apoptosis study and the flow cytometry were used to evaluate cell viability of mouse fibroblast L929.
After the hyperthermia test, the cells were incubated for 24 h prior to undergoing MTT assay to evaluate cell viability.
The remaining cell solutions in cultured 96-well plate were used to evaluate cell viability by 3- 4,5-dimethylthiazole-2-yl -2,5-diphenyl tetrazolium bromide (MTT) assay.
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