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Additionally, trace files were evaluated with the program Tracer vers.
Therefore, the likelihood of recombination among alleles was evaluated with the program Lamarc version 1.2.2 [ 41].
The energy of the crystallographic waters of the monomer was evaluated with the program DOWSER [ 54], keeping the waters that were located inside the pore.
For automated image analysis of digitised slides were accessed through the Slidepath Image Analysis system (Leica Microsystems GmbH, Wetzlar, Germany) and evaluated with the program's nuclear (for Ki-67), cytoplasmic (for CD68+) and membranous (for CD8+) scoring algorithm.
For automated image analysis for ERK5 immunoreactivity, digitised slides were accessed through the Slidepath Digital Image Hub (Dublin, Ireland) and evaluated with the program's nuclear scoring algorithm (Mohammed et al, 2012).
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PROPL has been evaluated with the programming languages Java and C and it has been reported that students who used this system were frequently better at creating algorithms for programming problems and demonstrated fewer errors in their implementation (Lane and VanLehn 2005).
For a given alignment, 20 model structures were built and evaluated with the programs PROCHECK [ 33] and ProSa [ 34] for stereo chemical and energetic validation, respectively, retaining only the model that better met the restrictions of both programs.
The putative functional relevance of the detected missense variations was evaluated with the programs: PolyPhen (http://genetics.bwh.harvard.edu/pph), SIFT (http://sift.bii.a-star.edu.sg/), SNAP (https://rostlab.org/services/snap/), pMUT (http://mmb.pcb.ub.es/PMut/) and MutPred (http://mutpred.mutdb.org/).mutdb.org/
Demographic factors will be evaluated with the statistics program SPSS v.16.
Amino acid conservation for a novel missense mutation was evaluated with the Conseq program (http://conseq.tau.ac.il/).
Samples were processed in a FACS-Calibur and evaluated with the CellQuest program.
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