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IS molecules were evaluated using Western blot analysis followed by computer-assisted densitometer evaluation.
Apoptosis and TRPV1 were evaluated using Western blot analysis.
Expression of AMPK, α1, α2, ACC, Akt, the Glut-4 translocation mediator Akt substrate of 160KD (AS160), mTOR, total and membrane Glut-4 was evaluated using Western blot.
Insulin receptor substrates (IRS-1, IRS-2) and post-receptor signaling molecules Akt, mammalian target of rapamycin (mTOR), ribosomal p70s6 kinase (p70s6k) and the eukaryotic translation initiation factor 4E (eIF4E -binding proteIF4E -binding as well as the aproteinc marker caspase-3 were evaluated using Western blot analysis.
Cells underwent I/R, and HSP 72 content was evaluated using Western blotting.
The biological activity of the oligonucleotides in complex with dendrimers was also evaluated using Western blot assays.
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The O-glycosylation, nuclear translocation, and transcriptional activation of heat shock factor-1 (HSF-1) and Sp1 were evaluated using immunoprecipitation, Western blotting, and luciferase assays.
17 Health status will be evaluated using the Western Ontario and McMaster Universities Osteoarthritis (WOMAC) Index.
Efficacy was evaluated using the Western Ontario and McMaster Universities (WOMAC) Osteoarthritis Index.
All band intensities were evaluated using ECL western blotting kit (Amersham Biosciences, Little Chalfont, UK) and were normalized to those of GAPDH.
Secondary measurements included range of motion (ROM), severity of knee pain (VAS), and a measure of perceived health and physical function, evaluated using the Western Ontario and McMaster Universities (WOMAC) Osteoarthritis Index.
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