The resulting sequences were then aligned and any mutations were evaluated using the Sequencher sequence alignment program (Gene Codes, Ann Arbor, MI, USA).
The similarity between non-identical amino acids was evaluated using the Gonnet sequence similarity matrix of the ClustalW program.
Cartilage volume and structural changes were evaluated using the following sequences: (1) three-dimensional spoiled gradient recalled sequence (SPGR) with fat suppression, (2) T1-weighted three-dimensional fast gradient recalled echo (T1w-GRE), and (3) T2-weighted fast spin echo sequence with fat saturation (T2w-FS).
Conditions for cycle sequencing were as follows: 1 cycle of 96°C for 5 min, 25 cycles of 96°C for 30 sec, 59°C for 15 sec, 60°C for 4 min. The products were separated by capillary electrophoresis (ABI 3100, Applied Biosystems, Foster City, CA) and evaluated using the software Sequencing analysis® (Applied Biosystems, Foster City, CA).
Transcript levels of osteogenic marker genes (ALP, COL1A1, SPP1/OPN, and RUNX2), adipogenic marker genes (FABP4/αP2, CEBPA/C/EBP-α, LEP/Leptin, and PPARG2/PPAR-γ2), and GAPDH (an internal standard) were evaluated using the primer sequences listed in Table 1.
Atypical amino acid residues in genital tract sequences compared with peripheral blood sequences were evaluated using the aligned protein sequences from the C2-V5 env region in the Viral Epidemiology Signature Pattern Analysis program (http://www.hiv.lanl.gov/content/hiv-db/P-vespa/vespa.html) [39].
Out of the six Musaceae species evaluated using the two different sequencing approaches, only M. textilis gave polymorphic ITS sequence after direct Sanger sequencing.
Under the optimized conditions, SFP detection performances in both genome and mRNA hybridizations were evaluated using the whole genome sequences of the two sequenced strains of O. sativa Nipponbare and 93-11.
Four RF coils were evaluated using the fourtypes of sequences (SE and FE with T1 and T2 weighting).
