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Ca2+ dynamics were evaluated using the changes in fluorescence intensity of calcium probe Fluo-3.
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This was evaluated using the change in PSD.
Our aim was to describe the relationship between Tdi, EXdi, TFdi and diaphragm function evaluated using the change in endotracheal pressure after phrenic nerve stimulation (Ptr,stim), and to compare their prognostic value.
Health-related QOL was evaluated using the change from baseline in the Incontinence Quality of Life (I-QOL) questionnaire total summary score [ 8].
Symptomatic and functional domains were evaluated using the change from baseline to the end of the double- blind dose-confirmation phase (week 9) total score on the physician-rated DSM-IV ADHD RS (range 0 54) [ 15] and self-rated SDS (range 0 30) [ 16] as co-primary endpoints.
The radiographic outcome at week 52 was evaluated using the mean change in total Sharp score (TSS) and the percentage of non-progressors (TSS change ≤0).
The behavior change stage to reduce screen time was also evaluated using the criterion of a behavior change stage of two hours daily [ 37].
These histopathological changes were evaluated using the Mankin score qualitatively.
The within-group changes were evaluated using the Wilcoxon signed rank test for statistical significance.
Relative expression levels were normalized to the housekeeping gene hypoxanthine guanine phosphoribosyl transferase 1 (HPRT1); expression changes were evaluated using the delta-delta Ct (ΔΔCt) method.
The responsiveness of the patient-reported outcomes to clinical change was evaluated using the two approaches discussed in the methods section.
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