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First- and second-order sensitivity coefficients of the thermal and mechanical response quantities (derivatives with respect to various thermomechanical parameters) are evaluated using a direct differentiation approach in conjunction with an automatic differentiation software facility.
Intracellular cAMP levels were evaluated using a direct cAMP enzyme immunoassay (Assay Designs, Ann Arbor, MI), per the manufacturer's recommendations, including optional acytelation of samples.
rhPRG4 and nhPRG4 binding to TLR2 and TLR4 was evaluated using a direct enzyme linked immunosorbent assay (ELISA).
Total and activated B cells in peripheral blood will be evaluated using a direct immune-fluorescence reagent (Fast Immune CD19/CD69/CD45, BD Biosciences).
The antibody samples were heated in a thermocycler at 85°C for different times up to 60 minutes, stored at 4°C for 1 hour, and then evaluated using a direct binding Luminex assay.
The size of the env gene open reading frame in the primate loci was evaluated using a direct coupled in vitro transcription/translation assay based on T7 promoter-containing PCR products as described in [ 24], which allows to determine the status of both alleles in the same assay.
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Protein expression was evaluated using a monoclonal antibody directed against the 6 × His tail.
Mechanical compressibility and shear strength of the collected MSW were evaluated using a 300 × 300 mm direct shear (DS) shear box.
Comparative toxicity across AgNO3 and the three different types of AgNPs was first evaluated using a C. elegans mortality test by a direct comparison of the LC50 values.
Life cycle impacts such as global warming, acidification, and eutrophication were evaluated using a life cycle assessment (LCA) framework, and direct water use was evaluated as the local impact.
Direct medical and nonmedical costs as well as indirect costs were evaluated using a structured questionnaire.
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CEO of Professional Science Editing for Scientists @ prosciediting.com