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The signals for β-actin were also evaluated to normalize protein loading.
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PCR efficiency could vary gene to gene, and should be evaluated in order to normalize qPCR data with the efficiency correction factor.
In this scenario, this paper proposes and then evaluates a method to normalize and expand original short and messy text messages in order to acquire better attributes and enhance the classification performance.
Beta actin, elongation factor 1 and ribosomal 18S genes were evaluated as reference genes to normalize the results before calculating the relative expression levels.
The expression of human HPRT was evaluated as a housekeeping gene to normalize the ΔCt values.
After 48 hours of incubation luciferase activities were evaluated, normalized to the transfection controls and the signaling intensities of EpCAM positive cells were then compared with the values obtained from the corresponding control cell lines.
A monoclonal Rat anti-HA (Roche 11 867 423 001, 1 200) antibody was used to evaluate total levels of expressed protein and to normalize pSMAD levels across multiple embryos.
A unique method of evaluating material refrigeration capacity is introduced and used to normalize material inputs.
In order to evaluate the amount of proteins, the optical density of the respective protein representing bands was evaluated and normalized to the optical density of the bands representing the housekeeping protein (Bio Photometer®, Eppendorf AG, Hamburg, Germany).
Here, we normalized vectors for RNA and p24 values prior to transduction and evaluated the transgene expression to determine the best titration method to normalize lentiviral vectors.
However, the potential for psychotherapy to normalize neurobiological responses to pleasant stimuli has not been evaluated.
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