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We first evaluated the specificity of the EGFP expression.
To validate our approach, we 1) compared in vivo measurements to ex vivo autoradiography and histology; 2) evaluated the specificity of tracer binding (in vivo displacement procedure); and 3) confirmed the presence of TSPO mRNA by means of in situ hybridization, as well as TSPO protein levels produced by the tumor by means of immunohistochemistry and western blotting, respectively.
In MCF-7 cells we also evaluated the specificity of the designed siRNAs.
Moreover, we evaluated the specificity of the anti-grouper β-defensin polyclonal antibody by Western blot and immunofluorescence respectively.
We evaluated the specificity of VA and inflammation.
We then evaluated the specificity of immunoaffinity purification.
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Further studies on a broader set of pathosystems are thus required to evaluate the specificity of the involvement of some specific defence mechanisms more accurately.
We used Nox4-specific siRNAs to knockdown endogenous Nox4 to evaluate the specificity of the Nox4 antibody.
HIVwt was also generated in parallel in the presence of the FlAsH dye (HIVwt-F) to evaluate the specificity of the labeling and the potential packaging of non-specifically labeled cellular proteins.
Drug free blank hair was analysed to evaluate the specificity of analytical method.
In order to evaluate the specificity of primer sets used for RT-qPCR amplification, the melting curve was analyzed.
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