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We have identified an interesting OXPHOS-dependent apoptotic mechanism via mtDNA upregulation, and therefore, have evaluated the requirement of this pathway for the efficacy of a wide array of anticancer agents.
However, these studies only evaluated the requirement of single genes for resistance to DNA-damaging agents, whereas the effects on genetic networks were only studied in a small directed screen (St Onge et al. 2007).
The majority of these factors had been identified in a prior screen that evaluated the requirement of chromatin regulators in MLL-AF9/NrasG12D acute myeloid leukemia (AML) (Zuber et al., 2011b), however TRIM33 was identified here as a unique requirement in B-ALL.
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Domain experts evaluated the requirements confirming the demand for more coordination along the patient's complete medical pathway.
We also evaluated the requirements of fluid, inotropic agents, blood transfusion and the intensive care unit (ICU) and hospital length of stay between groups.
We previously established His-tagged mouse IZUMO1 transgenic mouse lines with Izumo1−/− background to evaluate the requirement for IZUMO1 in fertility [10].
In this study, we used the Ub fusion model to determine whether wild type mature Smac maintains a proapoptotic function after prolonged cytosolic localization, and evaluate the requirement of the IBM to induce apoptosis [37], [38], [39].
To evaluate the requirement for TERT for primitive hematopoiesis in zebrafish, we analyzed early hematopoietic markers in the anterior lateral mesoderm (ALM) which produces myeloid cells, and the posterior lateral mesoderm (PLM) that produces erythroid cells [53].
The findings that TNF-α is involved with the leishmanicidal activity and that NO is released in the co-cultures led us to evaluate the requirement for nitrogen and oxygen radicals in L. (L).
Statistical models evaluating the requirement to treat (cumulative logit model) or the suppression of c-day 11 parasitemia (GLM) showed that both adjuvant (FA superior to ISA-720) and antigen (coli42-FVO superior to bv42-FVO) affected the induction of protective effects against erythrocytic stage FVO strain P. falciparum.
The lethality can be rescued by expression of AP2 components in the skin, which allowed us to evaluate the requirement for AP2 subunits at synapses.
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