We next evaluated sphere-forming ability of SP and non-SP cells grown in neurosphere conditions for 2 weeks.
Thus, a discussion on stemness as evaluated by sphere assays by necessity should incorporate studies and findings relevant to anchorage-independent growth and anoikis resistance of tumor cells.
This difference may be explained by the fact that Dittmer et. al. were examining the effects of MSC added to aggregates of spontaneously floating cells in monolayer serum-containing culture as compared to our study in which the effect of MSC was evaluated in spheres grown from single cells plated at clonal density in stem cell promoting serum-free media.
The time-dependent, self-collision probabilities are evaluated for slab, sphere and infinite cylindrical geometries.
SST k ω model was evaluated on a sphere model first by comparing it to a semi-experimental equation of the convective heat transfer coefficient.
The source charge is located at z = 4 Å. Figure 1 shows the relative error, with respect to the analytic solution, for the electrostatic potential due to the surface-boundary induced polarization charges only, evaluated along the sphere diameter passing through the point charge.
In addition, the impact of depleting PLK1 mRNA on tumor-initiating cells was evaluated using tumor sphere assays.
Moreover, its suitability for biomolecular simulations is evaluated using small spheres of water, hydration properties of small biological molecules, and configurational sampling of β-hairpin pentapeptide YPGDV.
We evaluated the PLC/PRF/5 sphere-forming cells for their stemness characteristics.
To determine if CD133+ STA-ET-8.2 cells are similarly capable of generating both CD133+ and CD133- daughter cells we evaluated clonally derived spheres generated from single CD133+ cells.
In this process, the full 3D vicinity of each sphere is evaluated by transforming the data to spherical coordinates relative to the respective sphere's center.
