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Cleavage and blastocyst formation were evaluated on days 2 and 7, respectively, with the day of SCNT designated day 0. Day 2 SCNT embryos were transferred to the oviducts of surrogates on the day of, or 1 day after, the onset of estrus.
Test solutions were applied on day 0 with ET function evaluated on days 1, 2, 3, and 7 after exposure.
The presence of an embryonic vesicle was detected on Day 25, embryonic/fetal movements and heartbeat were evaluated on Days 42 and 63 and embryo measurements [crown-rump length (CRL) and width at mid body] were obtained on Day 42 through ultrasonography.
These measurements were evaluated on days 3, 7, and 14 days after infection.
Additional predose samples (C (trough)) were evaluated on days 7, 21, and 35.
were evaluated on Days 9, 11, and 18. Cows from CIDR4 + CR treatment ovulated at 60 hours (53.3%) tended to be greater (P = 0.07) than CIDR4 + eCG (21.4%).
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Clinical parameters were evaluated on day 4 and survival was followed until day 14.
Retinal neovascularization was then evaluated on day P17 as described above.
Cell proliferation was evaluated on day 5 by measuring thymidine incorporation.
Cell proliferation was evaluated on day 4 post treatment with the CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, Mannheim, Germany).
Proliferation was evaluated on day 4 as a decrease of MFI within the positive population in FL-1 with responder cells only as positive control.
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