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The overall coverage of the four primer pairs was evaluated in silico with the Silva SSU r119 dataset.
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The possible impurities in the most frequently used topical excipients were evaluated in-silico for their toxicity hazard.
In current study, biotransformed products 2 7 were also evaluated in silico to understand their mode of interaction with the BChE.
This can be done through a number of computational tools with the outcomes evaluated in silico using flux balance analysis (FBA) [ 51] and further validated by in vivo experiments.
The specificity of these primer pairs were initially evaluated in silico using sequences obtained from GenBank and aligned with ClustalX (Table S1).
The putative antigenic variability in the 17 msp sequences was evaluated in silico by analyzing entire individual amino acid MSP sequences with CLC Bio Workbench [ 19].
SNPs with a statistically significant association were further evaluated in silico for potential function.
Potential functional effects of SNPs with at least one statistically significant association (p < 0.05) with U-Hg in at least one study group were evaluated in silico.
Primer specificity was evaluated in silico by BLAST analysis and agarose gel electrophoresis.
In addition, the cloned sequences were aligned with ClustalX [17] to the primers used for LH-PCR, and the virtual size of the corresponding fragments was evaluated (in silico analysis).
Each set was evaluated in silico to check that it ensured optimum coverage of the bacterial chromosome.
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