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By Blast search, we identified a total of 80 potential RNAi factors from the mined genome and transcriptome dataset of Sf21 cells and these factors were evaluated for validation using three factor-specific siRNAs that were transfected in the Sf21-gfp reporter cell line for gfp reversion [ 30].
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Various approaches were evaluated for model validation.
Both methods were also evaluated for crucial validation parameters.
It was of paramount importance to minimize the number of candidate biomarkers evaluated for further validation.
In total 192 SNPs were evaluated for SNP validation using the HRM assay (Additional file 6).
Runoff simulations using these 'informed' parameter sets were then evaluated for an independent validation period in terms of the Nash-Sutcliffe efficiency of the hydrograph and the mean absolute relative error of the flow-duration curve.
are used to test for univariate associations, and then those associations are evaluated for replication and validation in independent patient cohorts [ 2].
The method was evaluated for a number of validation characteristics (accuracy, repeatability and intermediate precision, limit of detection, limit of quantification and calibration range).
The same value was subtracted from the PI evaluated for all individuals in the validation dataset.
To answer this question, the antibody used by Zhang et al. [ 42] should be evaluated for specificity, similar to the validation of the antibodies performed here, and double stainings should be performed to irrefutably demonstrate expression by RGCs and astrocytes.
Hence, the behavior of the response categories should be evaluated for appropriate functioning in further validation studies, especially in a sample that includes a large number of chronically ill patients.
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