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Further the yeast isolates were evaluated for their pathogenicity and adherence to intestinal epithelial cells.
To assess the functionality of the novel test system, nine different MLH1 amino acid replacements were introduced in vivo and evaluated for their pathogenicity.
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Novel and rare variants were further evaluated for their potential pathogenicity based on the gene affected, the conservation of the altered nucleotide, the predicted amino acid (AA) change if the variant occurred within a polypeptide gene and the conservation index (CI) of the altered amino acid, and the presence of heteroplasmy.
They were also evaluated for their haemolytic activity and did not show hydrolysis of sheep blood in vitro, this being another desirable phenotype in terms of lack of infectivity and pathogenicity.
To date, several missense mutations in LRRK2 have been linked to autosomal dominantly inherited PD (e.g. R1441C/G, Y1699C, G2019S, I2020T), and many additional LRRK2 variants are being evaluated for conclusive pathogenicity or increased risk for PD [23].
The KEGG database does not contain complete information on the mycolic acid pathway (MAP), which is unique to mycobacteria and essential for their pathogenicity and survival.
The A/duck/Thailand/144/2005 (andK/TH/144/05) A/quail/Thailand/551/20052005 (A/Q/TH/551/05) viruses were isolated from western Thailand and tested for their pathogenicity in ducks (15 ).
Furthermore, S. intermedius and S. hyicus are well known for their pathogenicity in animals but not in humans [ 11].
Since not all identified variants will be causing FH, it is important that reported variants are tested for their pathogenicity.
Some bacteria such as coagulase-negative staphylococci have been increasingly acknowledged for their pathogenicity (von Eiff et al. 2006).
Thus, the genome sequence of these two species would present a unique opportunity for comparative analysis to understand the basis for their pathogenicity, and genomic incompatibility.
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