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High-throughput screening hits are evaluated for integrity and aggregation to ensure quality leads.
RNA samples were evaluated for integrity of 18S and 28S rRNA by ethidium bromide staining of 1 mg of RNA resolved by electrophoresis on a 1.0% agarose/formaldehyde gel.
Interviews are randomly audiotaped and evaluated for integrity reasons.
Primer pairs were evaluated for integrity by analysis of the amplification plot, dissociation curves, and efficiency of PCR amplification.
Each sample was evaluated for integrity by agarose gel electrophoresis; only the samples with intact 18S and 28S ribosomal RNA bands were used.
Tissues were evaluated for integrity, friability, bleeding, mucus, and thickness of biopsy (presence of sub-mucosa or not) under a dissecting microscope by two different technicians in blinded way (Table 1).
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The resulting helix was evaluated for stereochemical integrity using Procheck (14).
From each smear a total of 100 spermatozoa were evaluated for acrosome integrity.
The housing stock is being systematically evaluated for structural integrity and viability, and a significant number will not be viable.
These hypotheses are subsequently evaluated for biological integrity and experimental relevance and assembled into causal biological networks.
All oocyte-development stage-matched RNA samples per treatment were evaluated for RNA integrity using the 2100 BioAnalyzer (Agilent, Santa Clara, USA).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com