Purified DNA fragments were checked on 1.5-2% agarose gel stained with Gel-Red® dye solution, then evaluated for concentration by detecting absorbance at a 260 nm wave length at a Nano Drop device (Thermo Scientific®).
The quality and quantity of the RNA samples were evaluated for concentration and purity using a NanoDrop ND-1000 Spectrophotometer (NanoDrop; Wilmington, DE), and for integrity by gel electrophoresis using the FlashGel® RNA cassette system (Lonza; Rockland, ME).
After resuspension of RNA in nuclease-free water (Gibco), the samples were measured and evaluated for concentration and purity (260 nm/280 nm ratio) using a BioPhotometer (Eppendorf), in which values between 1.7 and 2.0 for 260/280 nm ratio were considered optimal.
Samples of kidneys, liver, longissimus lumborum, and triceps brachii were collected and evaluated for concentrations of 1,25- OH 2 1,25- OH 2
The matrix effect was then evaluated for concentrations around those previously obtained, using five matrix-matched 226Ra standards, with increasing concentrations of Ca (1, 2, 5, 10, and 20 μg g−1) and Mg (0.25, 0.5, 1.25, 2.5, and 5 μg g−1), and comparing them with a standard without Ca and Mg, at the same 226Ra concentration (27.5 fg g−1).
In this large epidemiological study, > 2,000 human blood samples were evaluated for concentrations of PCBs, PCDD/PCDFs, and p,p′-DDE (Kočan et al. 2004).
Its effectiveness was evaluated for the concentration of skim milk and a whey protein concentrate with 80% (w/w) protein on a dry-matter basis (WPC80).
The semen collected by urethral catheterization from eight male cats was evaluated for sperm concentration and motility and subsequently diluted with a TRIS-based extender to the concentration of spermatozoa 10 × 106/mL, 5 × 106/mL, and 1 × 106/mL.
The dose response was evaluated for fixed concentration of mitotic cyclin Clb2. Figure 5 shows the predicted response curve for the fractional Cln1/2 concentration, which varied depending upon the concentration of Clb2.
Several samples were evaluated for each concentration to the reproducibility of the measurements.
The tablet was further evaluated for plasma concentration of INF-β in the New Zealand White rabbit, and compared to a known subcutaneous formulation.
