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To identify cell types regulated by SCF and IGF-1 signals, we evaluated expression of the SCF receptor (c-Kit) and the IGF-1 receptor (IGF-1R) on erythroid cells in fetal spleen using flow cytometry (Fig. 5A,B).
Next, we evaluated expression of the proapoptotic protein BIM (Bcl2 interacting mediator of cell death) and the tumour suppressor p21 (Cdkn1A), both established targets of miR-17-92 miR-17-92 miR-17-928; Ventura et al, 2008; Xiao et al, 2008; Petrocca et al, 2008b).
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The virus was used to transfect cultured mouse endothelial cells (SVEC) and rat neonatal cardiac myocytes to evaluate expression of the protein by Western blotting.
In addition, they conducted an immunological and histological chemical study to evaluate expression of the MMP7 protein, whose gene was the one most overexpressed in normal tissue.
ELISA was performed to evaluate expressions of the DAG and IP3 as previously described [ 26].
We next evaluated expression and secretion of the T3sVHHs by Western blot (WB).
The Genevestigator database [ 37] was used to evaluate expression differences of the selected genes.
Although the expression of EGFR has not been evaluated in SBA, one study has evaluated the expression of the ErbB family member, HER2.
GFP positive cells were evaluated for expression of the surface proteins indicated.
We first evaluated the expression of the reprogramming factors, following the initial infection, as well as in the established Huv-iPS cell lines generated.
We then evaluated the expression of the chimeras using pAcSG2 and pcDNA3 plasmids in sf9 insect cells and in mammalian cell lines, respectively.
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