In order to evaluate dead cells (red) and viable ones (green), fluorescence was quantified using a Carl Zeiss Observer.D1 microscope (Oberkochen, Germany).
Cells were harvested at indicated time points, washed in PBS, resuspended in 1× binding buffer, and stained with 1 μl Annexin V (Biolegend) per 50 μl staining volume. 1 μl 7-AAD (Biolegend) per 50 μl staining volume was added to evaluate dead cells.
In this study we seek to evaluate dead-end centrifugation as a means of cell recovery and concentration and the effects upon cell quality as a result of the relative centrifugal force and time of centrifugation used.
Annexin-V-positive cells plotted in the lower and upper right quadrants were evaluated as dead cells.
The O/W separation performance was evaluated by dead-end filtration of a series of oil dispersion and emulsions.
The present study implements a three-part metric to evaluate the dead spot in terms of time, magnitude, and total area using center of pressure velocity and a novel threshold value calculation.
The permeate performance of this novel nanofiltration membrane was evaluated using a dead end filtration device, which gave us a high flux value for pure water (11.13 20.23 L m−2 h−1 bar−1) even under an extremely low external pressure (1.0 bar).
In the next experiment, we evaluated whether implanted dead Fe-Pro labeled hBMSCs would more readily transfer the label.
Here, (h_{j,0}) and (s_{j,0}) are the specific enthalpy and entropy evaluated at the dead-state temperature and pressure and the chemical composition of the (j th stream.
To assess the biocompatibility of the dispensing system, experiments were conducted using mouse 3T3-J2 fibroblast cells at various operation conditions, then cell viability and proliferation were evaluated using a live-dead assay and a cell counting kit assay, respectively.
