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To investigate potential phenotypic changes that solid stress induces in cancer cells, we evaluated cell proliferation and apoptosis in the spheroids.
Therefore, we evaluated cell proliferation, neurogenesis, astrogliogenesis, survival and neuronal maturation in the medial prefrontal cortex (mPFC) and the hippocampus (HIPP) during learning an operant conditioning task.
We modulated ERβ expression in REN and MSTO-211H MMe cell lines and evaluated cell proliferation and EGF receptor (EGFR) activation.
We evaluated cell proliferation using MTT and colony formation assays.
After 72 h we evaluated cell proliferation and cell cycle progression.
The MTT assay, which evaluated cell proliferation by measuring metabolic activity, showed similar results.
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To evaluate cell proliferation, Ki-67, a marker of proliferating cells, was immunolabeled in transverse frozen sections of rat brain, at 7 days after MCAO (Anti-Ki-67, 1 : 300, Abcam).
We evaluated gliogenesis subsequent to H/I using immunofluorescence methods as well as by evaluating cell proliferation within the SVZ and by fate mapping SVZ cells using replication deficient retroviruses.
The EdU detection kit (Beyotime, China) was used to evaluate cell proliferation.
Fibroblast culture was performed to evaluate cell proliferation on the copolymers films.
Methods: Three cancer cell lines with up- or down-regulation of RCC2 were used to evaluate cell proliferation, apoptosis, Rac1 signaling and sensitivity to a group of nine chemotherapeutic drugs.
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