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The quality of the pool was evaluated by sequencing 20 clones from the mutagenized DNA library.
Candidate genes were evaluated by sequencing the whole transcript including exon-intron boundaries and potential promoters in the parental lines of the HS mice.
Errors due to the analytical procedure were evaluated by sequencing 42 control clones obtained from an already cloned and sequenced HPRT segment.
The methylation status of each CpG site was quantitatively evaluated by sequencing multiple clones.
This gene was evaluated by sequencing the coding region and splice functions in normal and affected family members with familial atrial fibrillation.
VEGFR2, PDGFRα and PDGFRβ mutations were evaluated by sequencing their tyrosine kinase domains in 8 CRC cell lines and in 92 samples of patients with CRC.
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The amplicons were evaluated by sequence clone libraries and phylogenetic analyses.
The respective subtypes or cardiotropic viruses can be evaluated by sequence analysis.
Only one K. pneumoniae isolate was evaluated by sequence analysis because all three of the K. pneumoniae isolates showed the same enzymes on the IEF gel (Table 2).
Closer examination of some of the cases indicated that this can often be explained by the fact that the ohnolog is actually in an early stage of pseudogenization through gradual decay of its coding sequence, as evaluated by sequence alignment.
For each mappable sequence between 16 and 28 nt and with 5 counts or above, hairpin folding was evaluated by sequence analysis to identify the presence of a stem loop using the RNA secondary structure prediction tool in the CLC Genomics Workbench.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com