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For survivin and COX-2, staining in the nucleus and cytoplasm was evaluated by scanning of the whole section and counting more than 1000 representative cells.
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Specific localization of the motifs was evaluated by scanning for each motif in the core ± 100.
Interobserver reliability was evaluated by scanning 148 joints of 14 patients, including 7 patients fulfilling ACR criteria of RA [ 12], successively by both investigators, who were blinded for each other's findings and all other study data.
The reproducibility of the method was evaluated by scanning the lower end of calibration curve's concentration ten times and calculating the coefficient of variation or relative standard deviation (RSD) and the results found were 3, 3 and 4% for caffeine, theobromine and trigonelline, respectively.
The effect of process variables on surface morphology of polymers was evaluated by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM).
Differentiation was evaluated by scanning electron microscopy, whereas the expression of bone markers such as ALP, Osteocalcin, COLL1 and Osteonectin was investigated by quantitative real time polymerase chain reaction (qRT-PCR).
The morphology of composites was evaluated by scanning electron microscopy (SEM) using Quanta 3D FEG (FEI Co .. Physicochemical properties chracteristic of electrical double layer (EDL) of nano-hydroxyapatite [Ca10(OH)2(PO4)6] surface were studied by means of potentiometric titration and electrophoretic measurements.
The structure and morphology of 3DTNEEs were evaluated by scanning electron microscopy.
In addition, the hydrogel interior morphology as well as porosity of structure was evaluated by scanning electron microscopy (SEM).
Particle characterization and size measurement – as the most important parameters in aerodynamic behavior – were done by the laser diffraction method, the surface morphology of microparticles was evaluated by scanning electron microscopy (SEM).
Results of IHC were evaluated by scanning each slide under low magnification to identify regions containing positive immunoreactivity.
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