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Evidence of thrombin generation can be evaluated by quantification of prothrombin fragment 1 + 2 (F1 + 2) and/or thrombin antithrombin (TAT) complexes.
The formation of the osteoblast phenotype was evaluated by quantification of osteoblast-related marker genes using real-time polymerase chain reaction (RT-PCR).
Cell proliferation in the HIPP was evaluated by quantification of PCNA-IR cells in two different times: the same day or seven days after the last experimental procedure.
Finally, apoptotic cell death was evaluated by quantification of condensed nuclei stained with Hoechst 33258.
MB levels were evaluated by quantification of tetramethylthioninium-ion (TMT-ion) concentrations using liquid chromatography - mass spectrometry (LC-MS/MS).
Induction of apoptosis was evaluated by quantification of caspase 3 and caspase 7 activities in treated and untreated COS only.
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The quality of DNAs was evaluated by quantification (Picogreen) and PCR amplification of fragments of 100-200bp in length.
The efficiency of gene silencing in the flowers and corolla of these lines was evaluated by the quantification of HD20 transcript levels at different developmental stages.
The cytotoxic potential of the biomaterials on ASCs was evaluated by spectrophotometric quantification of the LDH released in culture medium.
The cytotoxic potential of samples A, B, C, and D was evaluated by spectrophotometric quantification of the LDH enzyme release in the culture media by the embedded ASCS.
The system is based on a 96-well format, and the digestibility is evaluated by the quantification of reducing sugars using a MBTH based technique.
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