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The formation of the osteoblast phenotype was evaluated by quantification of osteoblast-related marker genes using real-time polymerase chain reaction (RT-PCR).
Evidence of thrombin generation can be evaluated by quantification of prothrombin fragment 1 + 2 (F1 + 2) and/or thrombin antithrombin (TAT) complexes.
Cell proliferation in the HIPP was evaluated by quantification of PCNA-IR cells in two different times: the same day or seven days after the last experimental procedure.
Finally, apoptotic cell death was evaluated by quantification of condensed nuclei stained with Hoechst 33258.
MB levels were evaluated by quantification of tetramethylthioninium-ion (TMT-ion) concentrations using liquid chromatography - mass spectrometry (LC-MS/MS).
The quality of DNAs was evaluated by quantification (Picogreen) and PCR amplification of fragments of 100-200bp in length.
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The activation of cells and parasiticidal activity were evaluated by mediator quantification and direct counting of infected macrophages.
Cellular growth was evaluated by protein quantification using the DC Protein Assay Kit (Bio-Rad, Hercules, Calif .. Total intracellular metal contents were quantified by inductively coupled plasma-mass spectroscopy (ICP-MS) at distinct phases of growth.
Spheroid viability was evaluated by ATP quantification after 72 h compound treatment.
The cytotoxic potential of the biomaterials on ASCs was evaluated by spectrophotometric quantification of the LDH released in culture medium.
HER2 overexpression was evaluated by relative quantification using TATA-binding protein as endogen control (Bossard et al, 2005).
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