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Protective effect of both plant extracts against hydrogen peroxide-induced oxidative stress in a mouse fibroblast-like NCTC cell line was evaluated by Neutral red and lactate dehydrogenase (LDH) methods, light microscopy and flow-cytometry.
AoSMCs proliferation was evaluated by neutral red assay.
Cell viability was evaluated by neutral red uptake (NRU) according to Borenfreund and Puerner [ 36], modified by NICEATM-ICCVAM [ 37].
A recent multireader exercise conducted under the auspices of Outcome Measures in Rheumatology concluded that each method discriminated adequately between anti-TNF and placebo treatment groups, although consistency and reliability was better with the SPARCC method, particularly when evaluated by neutral observers with limited experience in either method [ 50].
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The procedures and data were vetted and evaluated by two neutral evaluators who were not involved in teaching of the classes: the third author, a university faculty member who supervised the first two authors; and another humanities teacher with experience in action research in the same specialized school with more than 15 years of teaching experience.
The procedures and data were vetted and evaluated by two neutral evaluators who were not involved in teaching of the classes: the third author, a university faculty member who supervised the first two authors; and another humanities teacher with experience in action research in the same specialized school with more than 15 years of teaching experience. .
The proportions of living cells were evaluated by the neutral red (NR) uptake assay.
Corrosion resistance evaluated by means of neutral salt spray test showed that zinc nickel CMM coatings were more corrosion-resistant than the monolithic coating of zinc or nickel of similar thickness.
The compound induced DNA damage evaluated by the alkaline, neutral, and pH 12.1 version of the comet assay.
The number of living cells was evaluated by automated counting and neutral red assay as described in Repetto et al (2008).
Adipogenic differentiation was evaluated by the cellular accumulation of neutral lipid vacuoles that were stained with Oil Red O (Sigma-Aldrich).
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