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(c) Treatment induced apoptosis was evaluated by labeling with anti-phosphatidylserine (anti-PS) superparamagnetic antibodies and measuring relaxivity in NMR.

(d) Treatment induced necrosis was evaluated by labeling with anti-genomic DNA (anti-gDNA) superparamagnetic antibodies and measuring relaxivities in NMR.

RG cell morphology was evaluated by labeling sections for Blbp and RC2.

The proliferation of iNSPCs was also evaluated by labeling of nestin-positive cells with bromodeoxyuridine (BrdU), as per a previous report.

DNA nuclear condensation was evaluated by labelling the cells with 8 μM Hoechst 33342 for 60 min at 37°C.

For a shorter period of time of BCG exposure (6 h), apoptotic status of cells was evaluated by labelling with Annexin V (Sigma-Aldrich) as indicated by manufacture instructions.

The presence of correct FVII transcripts associated to the IVS6 + 1G > T mutation were evaluated by labelling of RT-PCR products (Fig. 2A).

Protein concentrations were evaluated by label-free MS1 intensity-based quantification with the use of the Progenesis LC-MS (Nonlinear Dynamics, Durham, NC, USA) software package, which estimated correlation of tryptic peptidogenicity and protein expression levels.

TOPO-II α detection was evaluated by a labelled polymer The En Vision-mouse + System-HRP System (DAKO, Carpinteria, CA, USA) was used.

ER and PR immunohistochemical labeling was evaluated by recording labeling intensity, location and estimated percentage of positive cells, based on evaluation of a minimum of twenty high power (400×) fields.

The adsorption and exchange are evaluated by fluorescent labeling of these proteins.