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We have evaluated binding of the anti-VCAM-1 Ab-conjugated PS liposomes to VCAM-1 using two in vitro models, as well as assessing the ability of these liposomes to catalyze blood coagulation reactions.
We calculated regional time activity curves in the tissue (tTACs) from the dynamic data and ROIs, and we evaluated binding of [11C]TMSX to A2ARs in the striata as a DVR using tTACs, a graphical analysis with the cerebral cortex as the reference region [45].
In consideration of turnover of intrinsic CD20, we evaluated binding of rituximab to the transfectants 7 days after virus infection.
Similar experiments in a flow chamber model evaluated binding of spherical particles ranging from 100 nm to 10 μm coated with endothelial ligand.
To place our findings in context with previous reports demonstrating that the valency of Fc-containing compounds affects interactions with their cognate receptors [ 16], we evaluated binding of the individual fractions of 2A-2HC with FcγRIIb, FcγRIII, and SIGN-R1 by SPR, and with FcγRIIb and FcγRIII by Biolayer Interferometry (see Additional File 1, Figure S1).
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Studies evaluating binding of trastuzumab to effector cells demonstrated the highest affinity of FcγRIIIA in primary NK and NK-92 (V-158) cells.
1H NMR spectroscopy was used to evaluate binding of the oxoanions NO3−, ReO4−, CF3SO3−, H2PO4−, HSO4−, CH3COO− and C6H5COO− to the receptor.
However, when evaluating binding of the recombinant Ficolins added in equal concentrations, rFicolin-3 still appeared to have a better binding capacity to acBSA at the applied assay conditions compared to rFicolin-2.
To evaluate binding of β-DG to the GST-SH3 domains, myoblast lysate prepared in radio immune-precipitation assay (RIPA) buffer was passed over a GST-SH3 domain affinity column.
We used ITC to evaluate binding of dSfmbt to methylated histone-tail peptides.
Concentration-dependent DNase I footprinting was used to evaluate binding of 2 and 3 to DNA sequences 2 5.
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