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Patency was evaluated at days 1, 5 7, 30 and 90.
Mice lacking both type I and type II IFN receptor signaling (IFNAR-/-/IFNGR-/) on either the 129/SvEv or C57BL/6J genetic background were evaluated at days 0, 6, 8, and 12 of infection.
Glucocorticoid receptor (GCR) number and the effective concentration of dexamethasone resulting in a 50% reduction of leukemic cell viability in vitro (EC-50) were evaluated at days 0 and 3.Increasing dexamethasone doses resulted in greater marrow blast response (P =.007), with a similar trend in peripheral-blood blast response.
Parasitological cure was also evaluated at Days 14 and 42 as a secondary outcome.
Tumor size was evaluated at days 0, 7, 13, 16 and 20.
The evolution of skin pigmentation was evaluated at days 14, 28, 56, and 84.
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CBC, biochemical profile and urinalysis were evaluated at Day 0, and then weekly (pre-operatively at Day 8 and Day 15) to define safety.
In the present study, platelet levels were evaluated at day 6 p.i, a time point at which levels were lowest and the percentage of surviving animals maximal.
Virus-specific antibody-secreting cells (ASC) in the lungs and spleens of mice were evaluated at day 4 post-challenge (with 5xLD50 PR8) by a modification of the ELISPOT assay[28].
CD8+ T cell responses were evaluated at day 14 post-infection (p.i .. Anti IL-7 antibody (M25) was a kind gift from Amgen.
7) and CD8+ T cell responses were evaluated at day 14 p.i.TLA was extracted from RH strain of parasite and preparation was made as previously described [14].
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