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Embryos were evaluated and samples of culture medium collected and frozen prior to assay for glycosidases at day 7 of culture.
The survival rate of eggs/larvae was evaluated, and samples were collected for antioxidant system analysis (catalase – CAT, glutathione transferase – GST, glutathione reductase – GR, and lipoperoxidation – LPO), and neurotoxic evaluation (cholinesterase – ChE).
The fraction and intensity of p16INK4a positive cells was evaluated and samples with strong p16INK4a staining in >70% positive cells were considered as p16INK4a positive (p16INK4a+) 15.
For each sample, the normalised melting curves were evaluated and samples were compared with a control consisting of genomic DNA from the LNCaP cell line (wild-type EGFR) in a deduced difference plot.
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The subjects were evaluated and tissue samples obtained in the Weill Cornell NIH General Clinical Research Center and the Department of Genetic Medicine Clinical Research Facility under an Institutional Review Board-approved clinical protocol.
Core body temperature, characteristics of joint pain and rash were evaluated and blood samples taken at each visit.
Relative gene expression was evaluated and tumor samples were then spatially distributed according to the expression of the trios of genes: PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2.
BALF samples (5 to10) per time point were evaluated, and each sample was assayed in triplicate.
With automatic ventilation, Pplat was regularly evaluated and discrete (sampling) control of the Pplat value is implemented.
The results from the ABI3100 Prism Genetic Analyzer were manually evaluated and each sample was read in duplicate.
The results were evaluated and the sample size of five found to achieve a significance level P = 0.05 and a power of 90%.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com