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In order to evaluate the amount of proteins, the optical density of the respective protein representing bands was evaluated and normalized to the optical density of the bands representing the housekeeping protein (Bio Photometer®, Eppendorf AG, Hamburg, Germany).
Once stretch has been evaluated and normalized, the split BNX files are merged into a single file (Fig. 2 e).
Data were evaluated and normalized using the Perseus software (Version 36, Max Planck Institute of Biochemistry, Martinsried, Germany).
The expression level of genes were evaluated and normalized using the reads per kilobase of transcript per million reads mapped method [ 77].
The time to recovery of a train-of-four (TOF) ratio of 0.9 was evaluated and normalized by the baseline TOF ratio to improve the accuracy of NMB data [ 13].
In order to limit the variations of the obtained weighting, the RTL score (V RTL x,y,z,t)) was evaluated and normalized as in Eq. (7).
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Gene expression was evaluated on the basis of the threshold cycle (CT value) and normalized to the expression of the GAPDH gene.
Light emission was evaluated by luminometer, and normalized to a β-galactosidase luciferase reference plasmid.
The change in GFP and DsRed fluorescence intensity was evaluated inside mitochondria and normalized to the whole cell fluorescence intensity to contemplate photobleaching.
On the contrary, Oat5 urinary excretion, evaluated by Western blotting technique and normalized with the concentration of creatinine in urine, was increased by 111%.
The Ct values for each of the target genes evaluated were normalized with GAPDH Ct values for each sample.
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