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To evaluate whether the increase in DNA binding observed in the presence of AICAR was the result of increased protein expression, we measured USF-1 and GATA-4 protein by western blotting.
To further evaluate whether the increase of miR-21 also increased VEGF expression at mRNA level, the expression of VEGF and GAPDH was tested by semi-quantitative RT-PCR and real-time RT-PCR.
In a sensitivity analysis, we excluded dilute urine samples to evaluate whether the increase in iodine status across gestation was a result of dilution bias.
In addition, to evaluate whether the increase in SO42- incorporation was distributed evenly in the cartilage or incorporated in a more focal fashion, autoradiography was performed.
To evaluate whether the increase in rf is coordinated with the selection of new combinations of alleles of relevant tolerance genes, these results should be complemented with fine-scale assays of recombination landscapes and genome scanning for footprints of selection.
To evaluate whether the increase in apoptosis and ER volume density was caused by metabolic stress, human islets were exposed to the saturated FFA palmitate for 48 h (Cnop et al., 2014, Cunha et al., 2008).
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We did not specifically evaluate whether the increased ultrasound numbers translated into improved image acquisition or interpretation or whether the scans were clinically relevant or changed patient management.
The purpose of this study was to evaluate whether the increased risk of placental abruption among women with chronic hypertension is modified by ischemic placental disease, specifically pregnancy-induced hypertension (PIH) and fetal growth restriction (FGR).
To evaluate whether the increased MCP-1 secretion was functional, macrophage migration was assayed in vitro.
Support vector machines were selected to evaluate whether the increased accessibility of the RSA-based methods involves a trade-off in accuracy.
To evaluate whether the increased dehiscence rate in the control group for this study is statistically significant (12.5 % in the control group and 0%% in the NPWT group) at least 31 animals would be required in each group.
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