We used Nox4-specific siRNAs to knockdown endogenous Nox4 to evaluate the specificity of the Nox4 antibody.
At the end of the assay, a melting curve was constructed to evaluate the specificity of the reaction.
To evaluate the specificity of seeding in human samples, CSF from 10 subjects with mixed dementia was also tested in the cell-free lysate assay.
To further evaluate the specificity of all decoy oligonucleotides, two splicing targets of each splicing factor were chosen; FMNL3 and NUMA1 for RBFOX1/2, RTN4, and SNAP91 for PTBP1 and USP8 and U2AF1 for SRSF1.
To further evaluate the specificity of FUS XRCC1 interaction, we performed a broad domain mapping analysis and identified that Glycine-rich region (aa268 aa355) of FUS is the major region involved in XRCC1 binding (Supplementary Fig. 3).
To evaluate the specificity of the carrier, we purified primary cells from the endothelium of adipose tissue.
Single and mixed pathogen cDNA/DNA templates were used to evaluate the specificity of the GeXP-multiplex assay.
To evaluate the specificity of long dsRNAs used in high-throughput RNA interference (RNAi) screens performed at the Drosophila RNAi Screening Center (DRSC), we performed a global analysis of their activity in 30 genome-wide screens completed at our facility.
Drug free blank hair was analysed to evaluate the specificity of analytical method.
In order to evaluate the specificity of primer sets used for RT-qPCR amplification, the melting curve was analyzed.
To evaluate the specificity of the cellular uptake of the FITC- MTX + PEG)-CS-NPs, FITC- MTXition experiments were carried out.
