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The comparative ΔΔCT method was used to evaluate the relative quantities of each amplified product in the samples.
The comparative ΔΔCt method was used to evaluate the relative quantities of each amplified product in the samples.
The comparative CT method was used to evaluate the relative quantities of each amplified product in the samples.
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The comparative ΔΔCT method was employed to evaluate the relative quantity of each amplified product in the samples.
The splicing index (SI), defined by Srinivasan K. et al [ 19], was then utilized to evaluate the relative quantity of splicing difference between two tissues.
We have also carried out a sensitivity analysis to evaluate the relative importance of some of the physical quantities involved in the simulations.
Those changes in mRNA levels due to transcription can then be compared with RNA stability measurements of the population to evaluate the relative contributions of each process to the accumulated quantity of mRNA [ 8].
β-actin served as control to evaluate the relative expression.
The Pfaffl analysis method was used to measure the relative quantity of gene expression [ 25].
We used the Pfaffl analysis method to measure the relative quantity of gene expression (Pfaffl, 2001).
Gene expression was evaluated by determining the relative quantity (ΔCT) of the mRNA using the gene expression analysis software provided with the iQ5 Cycler.
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