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We next performed the reporter-based assay to evaluate the functional consequence of the predicted piRNA:mRNA interactions.
To evaluate the functional consequence of Ank1 hemutationtion, we performed detergent extraction of band 3 from ghost membrane using Triton X-100.
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However, further investigations will be required to evaluate the functional consequences of these findings and the biochemical function of most of the analyzed miRNAs, which remains elusive.
45 CMR can evaluate the functional consequences of atheroma burden on the elastic function of arteries and can measure both regional and global aortic stiffness.
To evaluate the functional consequences of the thin myocardium seen in transgenic embryos, we used fetal echocardiography (Fig. 5H).
To evaluate the functional consequences of inhibition by TGF-ß, we examined the regulation PPAR-γ transcriptional activity.
To evaluate the functional consequences of manipulation of ZO-1 or ZONAB expression in RPE cells, we next assessed whether the changes in the RPE morphology lead to functional alterations by fluorescein angiography.
To evaluate the functional consequences of this deletion we performed a western blot on protein extracts from neural tissue of an affected dog and a healthy control dog to investigate whether the predicted longer mutant NDRG1 protein is expressed.
To evaluate the functional consequences of HKII redistribution on glucose metabolism, cells were subjected to a 20 to 30 min pre-incubation in the absence of glucose to maximize dissociation of HKII from mitochondria (Fig. 6B), and compared to cells with glucose present throughout (in which HKII remained mostly associated with mitochondria).
SNAP is a computational technique designed to evaluate the functional consequences of single AA residue substitutions.
To evaluate the functional consequences of ADAM activity, cell migration was assessed using scratch assays.
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