IHC was performed in all cases to evaluate the expression levels of the proliferation marker Ki-67, and to identify CD44-expressing cells, as potential CSCs.
The aim of this study was to evaluate the expression levels of DNA damage response (DDR) markers in potentially preneoplastic oral epithelial lesions (PPOELs).
Forward and reverse primers were designed to evaluate the expression levels of factors in hNPCs (GAPDH: sense, 5′-CCATGAGAAGTATGACAACAGCC-3′, and antisense, 5′-GGGTGCTAAGCAGTTGGTG-3′; cellular inhibitor of apoptosis 2 [cIAP2]: sense, 5′-CAACATGCCAAGTGGTTTCC-3′, and antisense, 5′-CTCCTGGGCTGTCTGATGTG-3′; and SOD2: sense, 5′-CTGGACAAACCTCAGCCCTA-3′, and antisense, 5′-TGATGGCTTCCAGCAACTC-3′).
To evaluate the expression levels of induced GFP-WT and GFP-NLSm fusion proteins, 20 µg of RIPA-soluble protein and a volume equivalent to 5.0 times the amount of the corresponding urea-soluble fraction was loaded on 10% SDS-polyacrylamide gel electrophoresis gels.
To evaluate the expression levels of induced GFP-WT and GFP-NLSm fusion proteins, iGFP-WT or iGFP-NLSm cells were plated in 6-MW plates (1.5 × 105 cells), and after 24 h, growth medium was replaced with pre-warmed growth medium with Dox (Dox+) or without Dox (Dox−) for an additional 72 h.
Samples were taken in appropriate time intervals during the fermentations to monitor the growth of bacterial cultures by measuring OD600 and wet cell weight (WCW) after centrifugation at 15,000 × g for 15 min at 4°C. 2,5-DKG reductase activities and the total intracellular protein concentrations were determined in order to evaluate the expression levels.
To evaluate the expression level of recombinant glucagon at a higher scale with controlled conditions, the WAVE bioreactor (GE Healthcare) was used at a scale up of 5 L (Figure 1A, lower lane).
(I) qRT PCR assay was used to evaluate the expression level of genes that are located at different distances away from pFind1 insertion sites in pFind1-iPSC lines and control OKS-iPSC lines.
In this approach, the ALP98 mouse mAb (directed against a linear epitope outside the mutated regions) [29] was used to evaluate the expression level of the mutated E1E2.
The SBC homemade human cDNA microarray (Shanghai, China) containing 15552 spots (including 768 controls and 14784 probes) was used to evaluate the expression level of 10379 known genes and 3022 ESTs.
To evaluate the expression level of neutral and autofluorescent bodies, a probe volume with xyz dimensions of 125 µm×125 µm×29 µm was defined at the mid-section of wild type or mutant worms.
